Detection of superoxide production in stimulated and unstimulated living cells using new cyclic nitrone spin traps
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چکیده
منابع مشابه
EPR Detection of the Superoxide Free Radical with the Nitrone Spin Traps DMPO and BMPO
acterizing free radicals in chemistry, biology and medicine, is detection by EPR spectroscopy. However, due to their high reactivity and short half-lives, direct EPR detection of many free radicals (e.g., superoxide, hydroxyl radical, alkyl radicals, etc.) is virtually impossible in solution at room temperature. Spin trapping is a technique developed in the late 1960s in which a nitrone or nitr...
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The chemistry of perfluoroisobutylene (PFIB) with nitrone and nitroso spin traps: an EPR/Spin trapping study.
While applying electron paramagnetic resonance (EPR)/Spin Trapping techniques, several reactive intermediate species were identified in the reaction of perfluoroisobutylene (PFIB) with nitrone and nitroso spin trap agents: the carbon dioxide radical anion (CO2.-), a carbonyl fluoride intermediate (COF), and vinyl carbanions of PFIB. The reaction of PFIB with N-t-butyl-alpha-phenylnitrone (PBN) ...
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ژورنال
عنوان ژورنال: Free Radical Biology and Medicine
سال: 2014
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2014.03.019